- The nucleus is absent in prokaryotes and the lack of this organelle is used as a basis to distinguish whether a cell is a prokaryote or a eukaryote. Only eukaryotes have a nucleus; prokaryotes lack a nucleus. However, prokaryotes have a region in their cell where the genetic material is located. This region is called a nucleoid. It is nucleus-like and not bound by a nuclear envelope that separates the genetic material from the cytoplasm.
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